Green Goblin (Norman Virgil Osborn) Created by writer Stan Lee and artist Steve Ditko, Green Goblin first appeared in The Amazing Spider-Man #14 (July 1964).
The Flash Created by writer Gardner Fox and artist Harry Lampert, the original Flash first appeared in Flash Comics #1 (January 1940)
Deathstroke the Terminator (Slade Wilson) Created by Marv Wolfman and George Pérez, Deathstroke first appeared in New Teen Titans (vol. 1) #2 (December 1980).
Kingpin (Wilson Fisk) Created by writer Stan Lee and artist John Romita Sr., Kingpin first appeared in The Amazing Spider-Man #50 (July 1967).
Colonel Nicholas Joseph "Nick" Fury Created by artist Jack Kirby and writer Stan Lee, Nick Fury first appeared in Sgt. Fury and his Howling Commandos #1 (May 1963).
Lois Lane (Lois Joanne Lane-Kent) Created by Jerry Siegel and Joe Shuster, Lois Lane first appeared in Action Comics #1 (June 30, 1938)
She-Hulk (Jennifer Susan Walters) Created by writer Stan Lee and artist John Buscema, she first appeared in Savage She-Hulk #1 (February 1980).
Blade (Eric Brooks) Created by writer Marv Wolfman and penciller Gene Colan, Blade first appeared in The Tomb of Dracula #10 (July 1973).
Mary Jane Watson Created by writer Stan Lee and artist John Romita, Sr., after a few partial appearances and references, Mary Jane Watson finally first fully appeared in The Amazing Spider-Man #42 (November 1966).
Galactus (Galan) Created by writer-editor Stan Lee and artist and co-plotter Jack Kirby, Galactus first appears in Fantastic Four #48 (March 1966).